Caveolae are small membrane invaginations in the cell membrane of fat and muscle cells that support the uptake of lipids into the cells. The project aims to investigate how caveolae change during the intake of high-energy diets and whether caveolae can contribute to the development of obesity. To this end, mice will be fed a highly enriched carbohydrate diet or a high-fat diet for up to 16 weeks, and the caveolae will be analysed by electron microscopy and at the gene and protein level in terms of number, structure and behaviour. The results will allow us to better understand how cellular membrane structures change during the development of obesity and how caveolae may accelerate this process.