Project description:
Non-contact manipulation of cells opens up new possibilities in tissue engineering. Through superparamagnetic iron oxide nanoparticles (SPIONs), cells can be made magnetically controllable. When comparing five different SPION systems, the magnetic steerability of the cells depended on the amount of cellularly ingested particles.
Using appropriate magnetic fields, we were able to seed the cells into cell culture dishes in a targeted manner. Magnetic loading and sedimentation accelerated the formation of multicellular spheroids from many thousands of cells. Simultaneous use of non-loaded and SPION-loaded cells resulted in polarity in the cellular spheroids as the SPION-loaded cells aligned on the side of the magnetic field. In the future, remote control of different "magnetized" cell types could be used to build multilayered tissues without the need for a scaffold structure.
Publication:
Kappes M, Friedrich RP, Pfister F, Huber C, Friedrich RP, Stein R, Braun C, Band J, Schreiber E, Alexiou C, Janko C.
Superparamagnetic Iron Oxide Nanoparticles for Targeted Cell Seeding: Magnetic Patterning and Magnetic 3D Cell Culture. Adv. Funct. Mater., 2022, 2203672
Paper: https://onlinelibrary.wiley.com/doi/10.1002/adfm.202203672
Submitting the Paper of the Month:
Else Kröner-Fresenius-Stiftung is using the series “Paper of the Month” to report in an exemplary and hot-off-the-press style on the outstanding work being done by the scientists it supports. We regularly present publications which have recently appeared in especially renowned journals, have emerged from foundation funding and been given appropriate acknowledgment. This is done in the respective categories “Original Paper” and “Review”. In the case of each of these publications, the first author and/or senior author are being funded by the foundation.
We invite all project managers, fellowship recipients and members of graduate study programs to send their work in accordance with the stated criteria as proposal for Paper of the Month to Ms. Anne Asschenfeldt (a.asschenfeldt@ekfs.de).
